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J Cell Sci. 2016 Jul 1;129(13):2514-25. doi: 10.1242/jcs.186460. Epub 2016 May 20.

Drosophila Ana1 is required for centrosome assembly and centriole elongation.

Author information

1
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
2
Micron Oxford Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
3
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK jordan.raff@path.ox.ac.uk.

Abstract

Centrioles organise centrosomes and cilia, and these organelles have an important role in many cell processes. In flies, the centriole protein Ana1 is required for the assembly of functional centrosomes and cilia. It has recently been shown that Cep135 (also known as Bld10) initially recruits Ana1 to newly formed centrioles, and that Ana1 then recruits Asl (known as Cep152 in mammals) to promote the conversion of these centrioles into centrosomes. Here, we show that ana1 mutants lack detectable centrosomes in vivo, that Ana1 is irreversibly incorporated into centrioles during their assembly and appears to play a more important role in maintaining Asl at centrioles than in initially recruiting Asl to centrioles. Unexpectedly, we also find that Ana1 promotes centriole elongation in a dose-dependent manner: centrioles are shorter when Ana1 dosage is reduced and are longer when Ana1 is overexpressed. This latter function of Ana1 appears to be distinct from its role in centrosome and cilium function, as a GFP-Ana1 fusion lacking the N-terminal 639 amino acids of the protein can support centrosome assembly and cilium function but cannot promote centriole over-elongation when overexpressed.

KEYWORDS:

Ana1; Centriole; Centriole elongation; Centriole structure; Centrosome; Cep295

PMID:
27206860
PMCID:
PMC4958303
DOI:
10.1242/jcs.186460
[Indexed for MEDLINE]
Free PMC Article

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