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J Microbiol Biotechnol. 2016 Aug 28;26(8):1428-38. doi: 10.4014/jmb.1603.03074.

Biosorption of Lead(II) by Arthrobacter sp. 25: Process Optimization and Mechanism.

Author information

1
College of Resource and Environment, Northeast Agricultural University, Harbin 150030, P.R. China.

Abstract

In the present work, Arthrobacter sp. 25, a lead-tolerant bacterium, was assayed to remove lead(II) from aqueous solution. The biosorption process was optimized by response surface methodology (RSM) based on the Box-Behnken design. The relationships between dependent and independent variables were quantitatively determined by second-order polynomial equation and 3D response surface plots. The biosorption mechanism was explored by characterization of the biosorbent before and after biosorption using atomic force microscopy (AFM), scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The results showed that the maximum adsorption capacity of 9.6 mg/g was obtained at the initial lead ion concentration of 108.79 mg/l, pH value of 5.75, and biosorbent dosage of 9.9 g/l (fresh weight), which was close to the theoretically expected value of 9.88 mg/g. Arthrobacter sp. 25 is an ellipsoidalshaped bacterium covered with extracellular polymeric substances. The biosorption mechanism involved physical adsorption and microprecipitation as well as ion exchange, and functional groups such as phosphoryl, hydroxyl, amino, amide, carbonyl, and phosphate groups played vital roles in adsorption. The results indicate that Arthrobacter sp. 25 may be potentially used as a biosorbent for low-concentration lead(II) removal from wastewater.

KEYWORDS:

Arthrobacter sp.; biosorption mechanism; lead(II); optimization of biosorption process

PMID:
27197671
DOI:
10.4014/jmb.1603.03074
[Indexed for MEDLINE]
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