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Proc Natl Acad Sci U S A. 1977 Dec;74(12):5421-5.

High resolution two-dimensional electrophoresis of human plasma proteins.

Abstract

The two-dimensional electrophoretic technique of O'Farrell has been adapted to the analysis of human plasma proteins, and 30 polypeptides have been identified in the pattern produced. Genetic variants involving charge (isoelectric point) or size (molecular weight in the presence of sodium dodecyl sulfate) changes should be routinely detectable in at least 20 proteins at once, facilitating studies of human mutation rates.

PMID:
271964
PMCID:
PMC431746
[PubMed - indexed for MEDLINE]
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