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Protoplasma. 2017 Mar;254(2):713-724. doi: 10.1007/s00709-016-0982-y. Epub 2016 May 19.

Low temperature caused modifications in the arrangement of cell wall pectins due to changes of osmotic potential of cells of maize leaves (Zea mays L.).

Author information

1
Department of Plant Biochemistry and Physiology, Plant Breeding and Acclimatization Institute - National Research Institute, Radzików, 05-870, Błonie, Poland. a.bilska@ihar.edu.pl.
2
Department of Plant Physiology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Werynia 502, 36-100, Kolbuszowa, Poland. a.bilska@ihar.edu.pl.
3
Department of Plant Molecular Ecophysiology, Faculty of Biology, Institute of Plant Experimental Biology and Biotechnology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
4
Biomedical Sciences Research Complex North Haugh, University of St. Andrews, KY16 9ST, St. Andrews, Fife, Scotland, UK.
5
Department of Plant Pathology, Plant Breeding and Acclimatization Institute - National Research Institute, Radzików, 05-870, Błonie, Poland.
6
Laboratory of Electron Microscopy, Nencki Institute of Experimental Biology, PAS, Pasteura 3, 02-093, Warsaw, Poland.

Abstract

The cell wall emerged as one of the important structures in plant stress responses. To investigate the effect of cold on the cell wall properties, the content and localization of pectins and pectin methylesterase (PME) activity, were studied in two maize inbred lines characterized by different sensitivity to cold. Low temperature (14/12 °C) caused a reduction of pectin content and PME activity in leaves of chilling-sensitive maize line, especially after prolonged treatment (28 h and 7 days). Furthermore, immunocytohistological studies, using JIM5 and JIM7 antibodies, revealed a decrease of labeling of both low- and high-methylesterified pectins in this maize line. The osmotic potential, quantified by means of incipient plasmolysis was lower in several types of cells of chilling-sensitive maize line which was correlated with the accumulation of sucrose. These studies present new finding on the effect of cold stress on the cell wall properties in conjunction with changes in the osmotic potential of maize leaf cells.

KEYWORDS:

Cell wall; Cold stress; Osmotic potential; Pectins; Zea mays

PMID:
27193139
PMCID:
PMC5309300
DOI:
10.1007/s00709-016-0982-y
[Indexed for MEDLINE]
Free PMC Article

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