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Int Urogynecol J. 2016 Nov;27(11):1729-1734. Epub 2016 May 18.

Urinary proteomic pattern in female stress urinary incontinence: a pilot study.

Author information

1
Clinical Division of General Gynecology and Gynecological Oncology, Department of Obstetrics and Gynecology, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
2
Core Facility Proteomics, Clinical Institute of Laboratory Medicine, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria.
3
Science Consult Thomas Mohr KG, Guntramsdorf, Austria.
4
Clinical Division of General Gynecology and Gynecological Oncology, Department of Obstetrics and Gynecology, Medical University of Vienna, Spitalgasse 23, 1090, Vienna, Austria. suiproteomics@gmail.com.

Abstract

INTRODUCTION AND HYPOTHESIS:

Previous studies aiming to identify specific pre-defined urine protein biomarkers for stress urinary incontinence (SUI) have not identified clinically important differences. The hypothesis of our study was that the global distribution of urinary proteins, the proteome, differs between women with and those without SUI.

METHODS:

In this age-matched case-control study, we compared the urinary proteome of 20 women with SUI and 20 controls. Proteins were identified by applying high-performance liquid chromatography separation and tandem mass spectrometry detection. Data analysis was performed using Mascot 2.4.1 embedded in ProteinScape 3.1.

RESULTS:

We identified 828 different proteins. The concentration of six of those showed a significant difference between urine samples of SUI patients and those of controls (q value < 0.25). Four proteins showed a higher abundance in SUI samples compared with controls: plasma serine protease inhibitor (logFC 1.11), leucine-rich alpha-2-glycoprotein (logFC 3.91), lysosomal alpha-glucosidase (logFC 1.24), and peptidyl-prolyl cis- trans isomerase A (logFC 1.96). We identified two proteins in lower abundance in SUI samples compared with controls: uromodulin (logFC -4.87) and TALPID3 (logFC -1.99).

CONCLUSIONS:

Overexpression of plasma serine protease inhibitor, leucine-rich alpha-2-glycoprotein, lysosomal alpha-glucosidase, and peptidyl-prolyl cis- trans isomerase A, and lower expression of uromodulin and TALPID3, in urine may be associated with female SUI.

KEYWORDS:

Leucine- rich alpha-2-glycoprotein; Lysosomal alpha-glucosidase; Plasma serine protease inhibitor; Stress urinary incontinence; TALPID3; Urinary proteome; Uromodulin

PMID:
27193112
PMCID:
PMC5065895
DOI:
10.1007/s00192-016-3033-5
[Indexed for MEDLINE]
Free PMC Article

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