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Oncotarget. 2016 Jun 21;7(25):38036-38051. doi: 10.18632/oncotarget.9342.

Notch1 pathway-mediated microRNA-151-5p promotes gastric cancer progression.

Hsu KW1,2, Fang WL3,4, Huang KH3,4, Huang TT1,5, Lee HC5, Hsieh RH6, Chi CW5,7, Yeh TS1,8,9,10.

Author information

1
Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
2
Present address: Research Center for Tumor Medical Science, China Medical University, Taichung 404, Taiwan.
3
Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
4
Department of Surgery, Taipei Veterans General Hospital, Taipei 112, Taiwan.
5
Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
6
School of Nutrition and Health Sciences, College of Public Health and Nutrition, Taipei Medical University, Taipei 110, Taiwan.
7
Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan.
8
Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan.
9
Genome Research Center, National Yang-Ming University, Taipei 112, Taiwan.
10
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.

Abstract

Gastric carcinoma is the third leading cause of lethal cancer worldwide. Previous studies showed that Notch1 receptor intracellular domain (N1IC), the activated form of Notch1 receptor, promotes gastric cancer progression. It has been demonstrated that a significant cross-talk interplays between Notch pathways and microRNAs (miRNAs) in controlling tumorigenesis. This study identified an intronic microRNA-151 (miR-151), which consists of two mature miRNAs, miR-151-3p and miR-151-5p, as a Notch1 receptor-induced miRNA in gastric cancer cells. Activation of Notch1 pathway enhanced expressions of miR-151 and its host gene, focal adhesion kinase (FAK), in gastric cancer cells. The levels of miR-151 in gastric cancer samples were higher than those of adjacent non-tumor samples. Activated Notch1 pathway induced CBF1-dependent FAK promoter activity. The ectopic expression of miR-151 promoted growth and progression of SC-M1 gastric cancer cells including cell viability and colony formation, migration, and invasion abilities. Activated Notch1 pathway could augment progression of gastric cancer cells through miR-151-5p and FAK. The mRNA levels of pluripotency genes, Nanog and SOX-2, tumorsphere formation ability, tumor growth, and lung metastasis of SC-M1 cells were elevated by activated Notch1 pathway through miR-151-5p. Furthermore, miR-151-5p could target 3'-untranslated region (3'-UTR) of p53 mRNA and down-regulate p53 level in SC-M1 cells. Mechanistically, Notch1/miR-151-5p axis contributed to progression of SC-M1 cells through down-regulation of p53 which in turn repressed FAK promoter activity. Taken together, these results suggest that Notch1 pathway and miR-151-5p interplay with p53 in a reciprocal regulation loop in controlling gastric carcinogenesis.

KEYWORDS:

FAK; Notch1 receptor; gastric carcinogenesis; miR-151; p53

PMID:
27191259
PMCID:
PMC5122370
DOI:
10.18632/oncotarget.9342
[Indexed for MEDLINE]
Free PMC Article

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