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FEMS Microbiol Lett. 2016 Jun;363(12). pii: fnw109. doi: 10.1093/femsle/fnw109. Epub 2016 Apr 22.

Characterization of three positive regulators for tetramycin biosynthesis in Streptomyces ahygroscopicus.

Author information

1
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China.
2
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenhe District, Wenhua Road 103, Shenyang, Liaoning 110016, PR China xiahz612@sina.com.

Abstract

Three putative regulatory genes, namely ttmRI, ttmRII and ttmRIII, which are present in the tetramycin (ttm) biosynthetic gene cluster, were found in Streptomyces ahygroscopicus Disruption of ttmRI, ttmRII or ttmRIII reduced tetramycin production, and their complementation restored production to varying degrees. Gene expression analysis of the wild-type (WT) and mutant strains through reverse transcriptase-polymerase chain reaction (RT-PCR) of the ttm gene cluster showed that the expression levels of most of the biosynthetic genes were reduced in ΔttmRI, ΔttmRII and ΔttmRIII Electrophoretic mobility shift assays demonstrated that TtmRI, TtmRII and TtmRIII bound the promoters of several genes in the ttm gene cluster. This study found that these three proteins are a group of positive regulators that activate the transcription of the ttm gene cluster in S. ahygroscopicus In addition, ΔttmRII had a reduced degree of grey pigmentation. Thus, TtmRII has a pleiotropic regulatory function in the tetramycin biosynthetic pathway and in development.

KEYWORDS:

LAL family; regulator; regulatory network; streptomyces; tetramycin

PMID:
27190158
DOI:
10.1093/femsle/fnw109
[Indexed for MEDLINE]

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