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Traffic. 2016 Sep;17(9):1054-7. doi: 10.1111/tra.12414. Epub 2016 Jun 13.

Use of BODIPY-Cholesterol (TF-Chol) for Visualizing Lysosomal Cholesterol Accumulation.

Author information

1
Faculty of Medicine, Department of Anatomy, University of Helsinki, 00014 Helsinki, Finland.
2
Minerva Foundation Institute for Medical Research, 00290 Helsinki, Finland.
3
MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, OX39DS Oxford, UK.

Abstract

Dipyrromethene difluoride-cholesterol (TopFluor-Cholesterol, TF-Chol) is a widely used cholesterol analogue due to its excellent fluorescence properties and considerable similarity with natural cholesterol in terms of membrane partitioning. However, the suitability of TF-Chol for detecting lysosomal cholesterol deposition has recently been questioned. Here, we highlight the fact that the method of lipid delivery and the analysis of time-point both affect the membrane distribution and labeling pattern of TF-Chol, similarly as with radiolabeled cholesterol. Lysosomal sterol accumulation characteristic to a lysosomal storage disease is most readily detected when the probe is introduced via the physiological route, i.e. as a sterol fatty acid ester in low-density lipoprotein particles. When administered to cells from solvent, lysosomal sterol sequestration becomes evident after an overnight equilibration between membranes.

KEYWORDS:

BODIPY-cholesterol; Niemann-Pick type C disease; TF-cholesterol; cholesterol accumulation; lipid imaging

PMID:
27187581
PMCID:
PMC4988379
DOI:
10.1111/tra.12414
[Indexed for MEDLINE]
Free PMC Article

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