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Food Chem Toxicol. 2016 Jul;93:138-44. doi: 10.1016/j.fct.2016.05.008. Epub 2016 May 13.

Effects of zinc oxide nanoparticles on human coronary artery endothelial cells.

Author information

1
School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan; Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: kjc@tmu.edu.tw.
2
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: kangyunlee68@gmail.com.
3
Institute of Labor, Occupational Safety and Health, Ministry of Labor, New Taipei City, Taiwan; School of Public Health, National Defense Medical Center, Taipei, Taiwan. Electronic address: chpan@mail.iosh.gov.tw.
4
School of Public Health, National Defense Medical Center, Taipei, Taiwan. Electronic address: lgh@ndmctsgh.edu.tw.
5
Department of Internal Medicine, Division of Cardiology, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: hspenos@yahoo.com.tw.
6
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: shu-chuan@tmu.edu.tw.
7
Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong; Shenzhen Municipal Key Laboratory for Health Risk Analysis, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China. Electronic address: kfho@cuhk.edu.hk.
8
Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan; School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: r92841005@ntu.edu.tw.

Abstract

Inhalation of zinc oxide (ZnO) metal fumes is known to cause metal fume fever and to have systemic effects; however, the effects of ZnO nanoparticles (ZnONPs) on the cardiovascular system remain unclear. The objective of this study was to investigate the cardiovascular toxicity of ZnONPs. Human coronary artery endothelial cells (HCAECs) were exposed to ZnONPs of different sizes to investigate the cell viability, 8-hydroxy-2'-deoxyguanosine (8-OHdG), interleukin (IL)-6, nitric oxide (NO), and regulation of cardiovascular disease-related genes. Exposure of HCAECs to ZnONPs resulted in decreased cell viability and increased levels of 8-OHdG, IL-6, and NO. Downregulation of cardiovascular-associated genes was observed in response to ZnONPs in HCAECs determined by qPCR, suggesting that the calcium signaling pathway, neuroactive ligand-receptor interaction, hypertrophic cardiomyopathy, dilated cardiomyopathy, and renin-angiotensin system are important affected pathways in response to ZnONPs. Furthermore, we observed a significant response of AGTR1 to ZnONP exposure in HCAECs. Our results suggest that ZnONPs cause toxicity to HCAECs, which could be associated with cardiovascular dysfunction.

KEYWORDS:

AGTR1; Inflammation; Nanoparticles; Nitric oxide; Oxidative stress

PMID:
27185063
DOI:
10.1016/j.fct.2016.05.008
[Indexed for MEDLINE]

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