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Saudi J Gastroenterol. 2016 May-Jun;22(3):234-9. doi: 10.4103/1319-3767.182460.

Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats.

Author information

1
Central Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
2
Department of Gastroenterology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, P.R. China.

Abstract

BACKGROUND/AIMS:

This study aimed to investigate the difference among the expression of serotonin receptors (5-HT3, 5-HT4, and 5-HT7receptors) in colonic tissue of chronic diarrhea rats.

MATERIALS AND METHODS:

A rat model of chronic diarrhea was established by lactose diet. The expression of 5-HT3, 5-HT4, and 5-HT7receptors in the colonic tissue was detected using immunohistochemistry, real-time PCR and Western blotting techniques.

RESULTS:

There is no significant difference on the protein expression of 5-HT3receptor between the normal group and the chronic diarrhea model group. The mRNA expression of 5-HT3receptor in the chronic diarrhea model group was significantly lower than that in the normal group (n = 10; P< 0.01). The protein and mRNA expression of 5-HT4receptor in the chronic diarrhea model group were significantly higher than those in the normal group (n = 10; P< 0.05, P< 0.01). On the contrary, the protein and mRNA expressions of 5-HT7receptor in the chronic diarrhea model group were significantly decreased compared with the normal group (n = 10; P< 0.01, P< 0.01).

CONCLUSIONS:

The results suggested the receptors of 5-HT4and 5-HT7may be involved in inducing diarrhea by lactose diet.

PMID:
27184643
PMCID:
PMC4898094
DOI:
10.4103/1319-3767.182460
[Indexed for MEDLINE]
Free PMC Article

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