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Emerg Infect Dis. 2016 Jul;22(7):1295-7. doi: 10.3201/eid2207.160326. Epub 2016 Jul 15.

Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses.

Abstract

Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.

KEYWORDS:

Zika virus; arboviruses; chikungunya virus; dengue virus; multiplex molecular diagnostics; real-time RT-PCR; vector-borne infections; viruses

PMID:
27184629
PMCID:
PMC4918162
DOI:
10.3201/eid2207.160326
[Indexed for MEDLINE]
Free PMC Article

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