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J Immunol. 2016 May 15;196(10):3993-4002. doi: 10.4049/jimmunol.1501261. Epub 2016 Apr 20.

Prolonged Intake of Dietary Lipids Alters Membrane Structure and T Cell Responses in LDLr-/- Mice.

Author information

1
European Molecular Biology Laboratory Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales 2052, Australia;
2
School of Medical Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia;
3
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, New South Wales 2522, Australia;
4
Australian and New Zealand Army Corps Research Institute, Concord Repatriation General Hospital, Concord, New South Wales 2139, Australia; and.
5
Institute of Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, Australia, St. Lucia, Brisbane, Queensland 4072, Australia.
6
European Molecular Biology Laboratory Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia; Australian Research Council Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales 2052, Australia; k.gaus@unsw.edu.au j.rossy@unsw.edu.au.

Abstract

Although it is recognized that lipids and membrane organization in T cells affect signaling and T cell activation, to what extent dietary lipids alter T cell responsiveness in the absence of obesity and inflammation is not known. In this study, we fed low-density lipoprotein receptor knockout mice a Western high-fat diet for 1 or 9 wk and examined T cell responses in vivo along with T cell lipid composition, membrane order, and activation ex vivo. Our data showed that high levels of circulating lipids for a prolonged period elevated CD4(+) and CD8(+) T cell proliferation and resulted in an increased proportion of CD4(+) central-memory T cells within the draining lymph nodes following induction of contact hypersensitivity. In addition, the 9-wk Western high-fat diet elevated the total phospholipid content and monounsaturated fatty acid level, but decreased saturated phosphatidylcholine and sphingomyelin within the T cells. The altered lipid composition in the circulation, and of T cells, was also reflected by enhanced membrane order at the activation site of ex vivo activated T cells that corresponded to increased IL-2 mRNA levels. In conclusion, dietary lipids can modulate T cell lipid composition and responses in lipoprotein receptor knockout mice even in the absence of excess weight gain and a proinflammatory environment.

PMID:
27183636
DOI:
10.4049/jimmunol.1501261
[Indexed for MEDLINE]
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