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World J Gastroenterol. 2016 May 14;22(18):4515-28. doi: 10.3748/wjg.v22.i18.4515.

Dissecting characteristics and dynamics of differentially expressed proteins during multistage carcinogenesis of human colorectal cancer.

Author information

1
Fang Peng, Song-Ping Liang, Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan Province, China.

Abstract

AIM:

To discover novel biomarkers for early diagnosis, prognosis or treatment of human colorectal cancer.

METHODS:

iTRAQ 2D LC-MS/MS analysis was used to identify differentially expressed proteins (DEPs) in the human colonic epithelial carcinogenic process using laser capture microdissection-purified colonic epithelial cells from normal colon, adenoma, carcinoma in situ and invasive carcinoma tissues.

RESULTS:

A total of 326 DEPs were identified, and four DEPs (DMBT1, S100A9, Galectin-10, and S100A8) with progressive alteration in the carcinogenic process were further validated by immunohistochemistry. The DEPs were involved in multiple biological processes including cell cycle, cell adhesion, translation, mRNA processing, and protein synthesis. Some of the DEPs involved in cellular process such as "translation" and "mRNA splicing" were progressively up-regulated, while some DEPs involved in other processes such as "metabolism" and "cell response to stress" was progressively down-regulated. Other proteins with up- or down-regulation at certain stages of carcinogenesis may play various roles at different stages of the colorectal carcinogenic process.

CONCLUSION:

These findings give insights into our understanding of the mechanisms of colorectal carcinogenesis and provide clues for further investigation of carcinogenesis and identification of biomarkers.

KEYWORDS:

Biomarker; Carcinogenesis; Colorectal Cancer; Proteome

PMID:
27182161
PMCID:
PMC4858633
DOI:
10.3748/wjg.v22.i18.4515
[Indexed for MEDLINE]
Free PMC Article

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