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Org Biomol Chem. 2016 Jun 15;14(24):5468-76. doi: 10.1039/c6ob00473c.

Site-directed spin labeling of proteins for distance measurements in vitro and in cells.

Author information

1
Department of Chemistry, Zukunftskolleg, and Konstanz Research School Chemical Biology, University of Konstanz, Universit├Ątsstra├če 10, 78457 Konstanz, Germany. malte.drescher@uni-konstanz.de.
2
Department of Chemistry and Chemical Biology, Technical University of Dortmund, Otto-Hahn-Str. 6, 44227 Dortmund, Germany. daniel.summerer@tu-dortmund.de.

Abstract

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy allows studying the structure, dynamics, and interactions of proteins via distance measurements in the nanometer range. We here give an overview of available spin labels, the strategies for their introduction into proteins, and the associated potentials for protein structural studies in vitro and in the context of living cells.

PMID:
27181459
DOI:
10.1039/c6ob00473c
[Indexed for MEDLINE]

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