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Antonie Van Leeuwenhoek. 2016 Aug;109(8):1091-100. doi: 10.1007/s10482-016-0710-9. Epub 2016 May 14.

Thalassospira australica sp. nov. isolated from sea water.

Author information

1
Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia. eivanova@swin.edu.au.
2
Universidad Miguel Hernandez, Apartado 18, 03550, San Juan de Alicante, Spain.
3
Swinburne University of Technology, PO Box 218, Hawthorn, VIC, 3122, Australia.
4
A.V. Zhirmunsky Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, Pal'chevskogo Str., 17, Vladivostok, Russian Federation, 690041.
5
Far Eastern Federal University, Vladivostok, Russian Federation, 690950.
6
G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku, 159, Vladivostok, Russian Federation, 690022.
7
Far Eastern Federal University, 8 Suhanova St., Vladivostok, Russian Federation, 690950.

Abstract

Two Gram-negative, non-pigmented, motile bacteria were isolated from a sea water sample collected at St. Kilda Beach, Port Philip Bay, Victoria, Australia. The two strains were found to grow between 4 and 40 °C, pH 5-10 and tolerate up to 10 % NaCl. A phylogenetic study, based on a 16S rRNA gene sequence analysis indicated that strains NP 3b2(T) and H 94 belong to the genus Thalassospira. The sequence similarity of the 16S rRNA gene between the two new isolates is 99.8 % and between these strains and all validly named Thalassospira species was found to be in the range of 95-99.4 %. The DNA-DNA relatedness between the two strains was found to be 80.2 %, while relatedness with other validly named species of the genus Thalassospira was between 53 and 65 %. The average nucleotide identity (ANI) and the in silico genome-to-genome distance (GGD) between the two bacteria and T. profundimaris WP0211(T), T. xiamenensis M-5(T), 'T. permensis' NBRC 106175(T) and T. lucentensis QMT2(T) was 76-82 % and 21-25 %, respectively. The results of phylogenetic and genomic analysis, together with physiological and biochemical properties, indicated that the two strains represent a new species of the genus Thalassospira. Based on these data, a new species, Thalassospira australica, is proposed with strain NP 3b2(T) (=KMM 6365(T) = JCM 31222(T)) as the type strain.

KEYWORDS:

Genomic taxonomy; Marine bacteria; New species; Phylogeny; Taxonomy; Thalassospira

PMID:
27180097
DOI:
10.1007/s10482-016-0710-9
[Indexed for MEDLINE]

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