Format

Send to

Choose Destination
Biomaterials. 2016 Aug;98:53-63. doi: 10.1016/j.biomaterials.2016.04.040. Epub 2016 May 3.

In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Ĉerenkov imaging.

Author information

1
Department of Radiology, Washington University, St. Louis, MO 63110, USA.
2
Department of Internal Medicine, Washington University, St. Louis, MO 63110, USA.
3
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA; Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA.
4
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA; Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA; Laboratory for Synthetic-Biologic Interactions, Texas A&M University, College Station, TX 77843, USA.
5
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
6
Department of Chemistry, Washington University, St. Louis, MO 63110, USA.
7
Department of Radiology, Washington University, St. Louis, MO 63110, USA; Department of Internal Medicine, Washington University, St. Louis, MO 63110, USA. Electronic address: brodys@wustl.edu.

Abstract

Nanoparticles (NPs) play expanding roles in biomedical applications including imaging and therapy, however, their long-term fate and clearance profiles have yet to be fully characterized in vivo. NP delivery via the airway is particularly challenging, as the clearance may be inefficient and lung immune responses complex. Thus, specific material design is required for cargo delivery and quantitative, noninvasive methods are needed to characterize NP pharmacokinetics. Here, biocompatible poly(acrylamidoethylamine)-b-poly(dl-lactide) block copolymer-based degradable, cationic, shell-cross-linked knedel-like NPs (Dg-cSCKs) were employed to transfect plasmid DNA. Radioactive and optical beacons were attached to monitor biodistribution and imaging. The preferential release of cargo in acidic conditions provided enhanced transfection efficiency compared to non-degradable counterparts. In vivo gene transfer to the lung was correlated with NP pharmacokinetics by radiolabeling Dg-cSCKs and performing quantitative biodistribution with parallel positron emission tomography and Čerenkov imaging. Quantitation of imaging over 14 days corresponded with the pharmacokinetics of NP movement from the lung to gastrointestinal and renal routes, consistent with predicted degradation and excretion. This ability to noninvasively and accurately track NP fate highlights the advantage of incorporating multifunctionality into particle design.

KEYWORDS:

Biodistribution; Cytotoxicity; Degradable nanoparticle; Gene expression; Lung; Čerenkov luminescence imaging

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center