Format

Send to

Choose Destination
Biomaterials. 2016 Aug;98:31-40. doi: 10.1016/j.biomaterials.2016.04.023. Epub 2016 Apr 26.

Influence of particulate and dissociated metal-on-metal hip endoprosthesis wear on mesenchymal stromal cells in vivo and in vitro.

Author information

1
Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin, 10117 Berlin, Germany.
2
Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
3
Trauma Surgery and Orthopedics, DRK Kliniken Berlin Westend, 14050 Berlin, Germany.
4
Julius Wolff Institute, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany; Berlin-Brandenburg School for Regenerative Therapies, Berlin, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany.
5
Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin, 10117 Berlin, Germany; Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany; Berlin-Brandenburg School for Regenerative Therapies, Berlin, Charité - Universitätsmedizin Berlin, 13353 Berlin, Germany. Electronic address: carsten.perka@charite.de.

Abstract

In hip arthroplasty the implants' articulating surfaces can be made of a cobalt-chromium-molybdenum (CoCrMo) alloy. The use of these metal-on-metal (MoM) pairings can lead to the release of wear products such as metallic particles and dissociated metal species, raising concerns regarding their safety amongst orthopedic surgeons and the public. MoM-wear particles are reported to be heterogeneous in their physicochemical properties, are capable of inducing adverse effects on a cellular level and are thought to be involved in relevant clinical problems like aseptic osteolysis. Yet, it remains elusive how MoM-wear affects bone forming cells and their progenitors: bone marrow residing mesenchymal stromal cells (MSCs). This study introduces an assessment of the in vivo exposure to particulate and dissociated Co and Cr and evaluates the effects of MoM-wear on MSCs. The exposure to MoM-wear products in vivo and in vitro leads to a decrease in MSCs' osteogenic matrix mineralization and alkaline phosphatase activity on a cellular and systemic level. In conclusion, MoM-wear products are released in the periprosthetic region and elevate bone marrow Co and Cr concentrations towards levels that impair osteogenic differentiation of MSCs. Therefore, the ongoing use of CoCrMo alloys for articulating surfaces in joint replacement implants needs critical reconsideration.

KEYWORDS:

Biocompatibility; CoCrMo alloy; Mesenchymal stromal cells; Metal toxicity; Metal-on-metal wear; Total hip arthroplasty

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center