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Biotechniques. 2016 May 1;60(5):252-9. doi: 10.2144/000114417. eCollection 2016.

Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells.

Author information

  • 1Inflammation Research Center, The Flanders Institute for Biotechnology (VIB), Ghent, Belgium.
  • 2Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • 3Department of Biomedical Molecular Biology, BCCM/LMBP Plasmid Collection, Ghent University, Belgium.

Abstract

In contrast to most common gene delivery techniques, lentiviral vectors allow targeting of almost any mammalian cell type, even non-dividing cells, and they stably integrate in the genome. Therefore, these vectors are a very powerful tool for biomedical research. Here we report the generation of a versatile new set of 22 lentiviral vectors with broad applicability in multiple research areas. In contrast to previous systems, our platform provides a choice between constitutive and/or conditional expression and six different C-terminal fusions. Furthermore, two compatible selection markers enable the easy derivation of stable cell lines co-expressing differently tagged transgenes in a constitutive or inducible manner. We show that all of the vector features are functional and that they contribute to transgene overexpression in proof-of-principle experiments.

KEYWORDS:

Gateway; Tet-On; co-inducible expression; lentiviral vectors

PMID:
27177818
DOI:
10.2144/000114417
[PubMed - in process]
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