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Parasit Vectors. 2016 May 13;9(1):278. doi: 10.1186/s13071-016-1562-x.

Hydatid cyst fluid promotes peri-cystic fibrosis in cystic echinococcosis by suppressing miR-19 expression.

Author information

1
Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
2
State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890298), The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
3
WHO-Collaborating Centre for the Prevention and Treatment of Human Echinococcosis, Department of Parasitology, University of Franche-Comté (EA 3181) and University Hospital, Besançon, France.
4
State Key Laboratory Incubation Base of Xinjiang Major Diseases Research (2010DS890298), The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. Dr.wenhao@163.com.
5
Xinjiang Key Laboratory of Echinococcosis, Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. renyong_lin@sina.com.

Abstract

BACKGROUND:

Echinococcus granulosus infection causes cystic echinococcosis (CE); the generation of liver fibrosis around the parasitic larval cyst (metacestode) may play a major role in the spontaneous limitation of the parasitic growth; however, fibrogenesis has received little attention in CE. It has been reported that miR-19b plays a role in various diseases, including infectious diseases, by regulating fibrogenesis. However, its function in the development of liver fibrosis in E. granulosus infection is unknown.

METHODS:

The expression of miR-19b and genes that are involved in liver fibrosis were analysed in E. granulosus-infected human livers using qRT-PCR. The role of miR-19b on hepatic stellate cells (LX-2 cells in vitro) treated with hydatid cyst fluid (HCF) was then analysed by 3-(4, 5-dimet-hylthiazol-2-yl)-2, 4-diphenyl-tetrazolium bromide (MTT) assay, qRT-PCR, Western blot and flow cytometry.

RESULTS:

The results showed that the expression of miR-19 was significantly reduced in the pericystic collagen-rich liver tissue of CE patients, compared to normal liver. Incubation of LX-2 cells (in vitro) with HCF induced a decreased proliferation of these cells and a reduced expression of miR-19, inversely correlated with the expression of collagen 1A1 and TGF-β receptor II (TβRII). Conversely, overexpression of miR-19 by LX-2 cells inhibited the proliferation of these cells and led to decreased TβRII expression.

CONCLUSIONS:

Our study provides new evidence for the intervention of miRNAs in the regulation of fibrosis in infectious diseases; it suggests that E. granulosus can inhibit miR-19 liver expression and promote fibrosis through the increase in TβRII, the activation of hepatic stellate cells and extracellular matrix production.

KEYWORDS:

Echinococcus granulosus; HSC; Liver fibrosis; TβRII; miR-19

PMID:
27177776
PMCID:
PMC4866024
DOI:
10.1186/s13071-016-1562-x
[Indexed for MEDLINE]
Free PMC Article

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