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Plant Signal Behav. 2016 Jun 2;11(6):e1183087. doi: 10.1080/15592324.2016.1183087.

An unbiased nuclear proteomics approach reveals novel nuclear protein components that participates in MAMP-triggered immunity.

Author information

1
a Department of Chemistry , Biochemistry and Physics and Groupe de Recherche en Biologie Végétale, Université du Québec à Trois-Rivières , Trois-Rivières , QC , Canada.

Abstract

(MAMP)-triggered immunity (MTI) is the first layer of molecular defense encountered by pathogens. Genetic screens have contributed to our knowledge of MTI, but are limited to phenotype-causing mutations. Here we attempt to identify novel factors involved in the early event leading to plant MTI by comparing the nuclear proteomes of two Arabidopsis genotypes treated with chitosan. Our approach revealed that following chitosan treatment, cerk1 plants had many nuclear accumulating proteins in common, but also some unique ones, when compared with Col-0 plants. Analysis of the identified proteins revealed a nuclear accumulation of DNA-modifying enzymes, RNA-binding proteins and ribosomal proteins. Our results demonstrate that nuclear proteomic is a valid, phenotype-independent approach to uncover factor involved in cellular processes.

KEYWORDS:

Arabidopsis; MAMP triggered immunity; cerk1; chitosan; nucleus; proteomic

PMID:
27177187
PMCID:
PMC4977454
DOI:
10.1080/15592324.2016.1183087
[Indexed for MEDLINE]
Free PMC Article

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