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World J Virol. 2016 May 12;5(2):85-6. doi: 10.5501/wjv.v5.i2.85.

Determination of 50% endpoint titer using a simple formula.

Author information

1
Muthannan Andavar Ramakrishnan, Division of Virology, Indian Veterinary Research Institute, Uttarakhand 263138, India.

Abstract

Two commonly used methods for calculating 50% endpoint using serial dilutions are Spearman-Karber method and Reed and Muench method. To understand/apply the above formulas, moderate statistical/mathematical skills are necessary. In this paper, a simple formula/method for calculating 50% endpoints has been proposed. The formula yields essentially similar results as those of the Spearman-Karber method. The formula has been rigorously evaluated with several samples.

KEYWORDS:

Endpoint dilution; Reed and Muench; Spearman-Karber; TCID50

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