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Cytometry B Clin Cytom. 2017 Sep;92(5):411-419. doi: 10.1002/cyto.b.21382. Epub 2016 Jun 28.

European canine lymphoma network consensus recommendations for reporting flow cytometry in canine hematopoietic neoplasms.

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Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado.
Immune Regulation Laboratory, Department of Clinical Science and Services, Royal Veterinary College, London, United Kingdom.
Department of Veterinary Sciences, University of Turin, Turin, Italy.
Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California-Davis, Davis, California.



Flow cytometry (FC) is assuming increasing importance in diagnosis in veterinary oncology. The European Canine Lymphoma Network (ECLN) is an international cooperation of different institutions working on canine lymphoma diagnosis and therapy. The ECLN panel of experts on FC has defined the issue of reporting FC on canine lymphoma and leukemia as their first hot topic, since a standardized report that includes all the important information is still lacking in veterinary medicine.


The flow cytometry panel of the ECLN started a consensus initiative using the Delphi approach. Clinicians were considered the main target of FC reports. A panel of experts in FC was interrogated about the important information needed from a report.


Using the feedback from clinicians and subsequent discussion, a list of information to be included in the report was made, with four different levels of recommendation. The final report should include both a quantitative part and a qualitative or descriptive part with interpretation of the salient results. Other items discussed included the necessity of reporting data regarding the quality of samples, use of absolute numbers of positive cells, cutoff values, the intensity of fluorescence, and possible aberrant patterns of antigen expression useful from a clinical point of view.


The consensus initiative is a first step toward standardization of diagnostic approach to canine hematopoietic neoplasms among different institutions and countries. This harmonization will improve communication and patient care and also facilitate the multicenter studies necessary to further our knowledge of canine hematopoietic neoplasms. © 2016 International Clinical Cytometry Society.


European canine lymphoma network; dog; guidelines; lymphoma; report

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