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Nature. 1989 May 18;339(6221):191-5.

Structural plasticity broadens the specificity of an engineered protease.

Author information

1
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.

Abstract

The substrate specificity of alpha-lytic protease has been changed dramatically, with a concomitant increase in activity, by replacing an active-site Met with Ala. The substrate specificity of both this mutant and another similar mutant are extraordinarily broad. X-ray crystallographic analysis shows that structural plasticity, a combination of alternate side-chain conformations and binding-site flexibility, allows both large and small substrates to be well accommodated.

PMID:
2716847
DOI:
10.1038/339191a0
[Indexed for MEDLINE]

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