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PLoS One. 2016 May 11;11(5):e0155311. doi: 10.1371/journal.pone.0155311. eCollection 2016.

YY1 Is Required for Germinal Center B Cell Development.

Author information

1
Department of Biomedical Sciences, University of Pennsylvania, School of Veterinary Medicine, 3800 Spruce Street, Philadelphia, Pennsylvania 19104, United States of America.
2
Department of Microbiology and Immunology, Thomas Jefferson University, 233 South Tenth Street, Philadelphia, Pennsylvania 19107, United States of America.

Abstract

YY1 has been implicated as a master regulator of germinal center B cell development as YY1 binding sites are frequently present in promoters of germinal center-expressed genes. YY1 is known to be important for other stages of B cell development including the pro-B and pre-B cells stages. To determine if YY1 plays a critical role in germinal center development, we evaluated YY1 expression during B cell development, and used a YY1 conditional knock-out approach for deletion of YY1 in germinal center B cells (CRE driven by the immunoglobulin heavy chain γ1 switch region promoter; γ1-CRE). We found that YY1 is most highly expressed in germinal center B cells and is increased 3 fold in splenic B cells activated by treatment with anti-IgM and anti-CD40. In addition, deletion of the yy1 gene by action of γ1-CRE recombinase resulted in significant loss of GC cells in both un-immunized and immunized contexts with corresponding loss of serum IgG1. Our results show a crucial role for YY1 in the germinal center reaction.

PMID:
27167731
PMCID:
PMC4863967
DOI:
10.1371/journal.pone.0155311
[Indexed for MEDLINE]
Free PMC Article

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