Format

Send to

Choose Destination
Chromosoma. 2017 Feb;126(1):45-58. doi: 10.1007/s00412-016-0600-y. Epub 2016 May 10.

Slx4 scaffolding in homologous recombination and checkpoint control: lessons from yeast.

Author information

1
Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, USA.
2
Department of Biosciences, University of Milan, 20133, Milan, Italy.
3
Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 14853, USA. mbs266@cornell.edu.

Abstract

Homologous recombination-mediated DNA repair is essential for maintaining genome integrity. It is a multi-step process that involves resection of DNA ends, strand invasion, DNA synthesis and/or DNA end ligation, and finally, the processing of recombination intermediates such as Holliday junctions or other joint molecules. Over the last 15 years, it has been established that the Slx4 protein plays key roles in the processing of recombination intermediates, functioning as a scaffold to coordinate the action of structure-specific endonucleases. Recent work in budding yeast has uncovered unexpected roles for Slx4 in the initial step of DNA-end resection and in the modulation of DNA damage checkpoint signaling. Here we review these latest findings and discuss the emerging role of yeast Slx4 as an important coordinator of DNA damage signaling responses and a regulator of multiple steps in homologous recombination-mediated repair.

KEYWORDS:

Checkpoint; Dpb11; Homologous recombination; Resection; Slx4

PMID:
27165041
PMCID:
PMC5104683
DOI:
10.1007/s00412-016-0600-y
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center