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Plant Physiol Biochem. 2016 Sep;106:159-64. doi: 10.1016/j.plaphy.2016.04.054. Epub 2016 Apr 30.

Pod removal responsive change in phytohormones and its impact on protein degradation and amino acid transport in source leaves of Brassica napus.

Author information

1
Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 500-600, Republic of Korea; Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
2
Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 500-600, Republic of Korea.
3
Central Instrument Facility, Gyeongsang National University, Jinju 600-701, Republic of Korea.
4
Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju 500-600, Republic of Korea. Electronic address: grassl@chonnam.ac.kr.

Abstract

To characterize the hormonal regulation of nitrogen remobilization from source to pod filling in Brassica napus, the hormonal level, proteolytic process, and amino acid transport were assessed in mature leaves of pod-removed or control at the early pod-filling stage. Pod (sink) removal decreased salicylic acid (SA), and significantly increased jasmonic acid (JA). The SA/JA ratio decreased with pod removal, accompanied by low degradation of foliar proteins and Rubisco content. A significant decrease in protease activity was observed in pod-removed leaves, confirmed by in-gel staining of protease. Pod removal reduced the expression of four amino acid transporter genes (BnAAP1, BnAAP2, BnAAP4, and BnAAP6) in mature leaves and reduced amino acid loading into phloem. These results indicated that a decrease in SA resulting from pod removal down-regulated nitrogen remobilization accompanied by a decrease in proteolytic activity and amino acid transport in mature leaves at the pod-filling stage.

KEYWORDS:

Amino acid transport; Brassica napus; Pod removal; Proteolysis; Salicylic acid

PMID:
27161582
DOI:
10.1016/j.plaphy.2016.04.054
[Indexed for MEDLINE]

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