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Sci Rep. 2016 May 10;6:25657. doi: 10.1038/srep25657.

Osthole inhibits histamine-dependent itch via modulating TRPV1 activity.

Author information

1
College of Basic Medicine, Nanjing University of Chinese Medicine, 138 XianLin Road, Nanjing 210023, China.
2
Jishou University, Jishou, 410023, China.
3
College of Life Science, Nanjing Normal University, Nanjing, 210046, China.
4
College of Basic Medicine, Guangxi University of Chinese Medicine, 13 WuHe Road, Nanning 530299, China.
5
The Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, China.

Abstract

Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as an antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied the molecular mechanism of osthole in histamine-dependent itch by behavioral test, Ca(2+) imaging, and electrophysiological experiments. First, osthole clearly remitted the scratching behaviors of mice induced with histamine, HTMT, and VUF8430. Second, in cultured dorsal root ganglion (DRG) neurons, osthole showed a dose-dependent inhibitory effect to histamine. On the same neurons, osthole also decreased the response to capsaicin and histamine. In further tests, the capsaicin-induced inward currents were inhibited by osthole. These results revealed that osthole inhibited histamine-dependent itch by modulating TRPV1 activity. This study will be helpful in understanding how osthole exerts anti-pruritus effects and suggests that osthole may be a useful treatment medicine for histamine-dependent itch.

PMID:
27160770
PMCID:
PMC4861971
DOI:
10.1038/srep25657
[Indexed for MEDLINE]
Free PMC Article

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