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Pharm Biol. 2016 Nov;54(11):2685-2691. Epub 2016 May 9.

Effect of mulberry leaf (Folium Mori) on insulin resistance via IRS-1/PI3K/Glut-4 signalling pathway in type 2 diabetes mellitus rats.

Cai S1, Sun W2,3,4, Fan Y5, Guo X6, Xu G6, Xu T7, Hou Y6, Zhao B8, Feng X9, Liu T2,3,4.

Author information

1
a Beijing University of Chinese Medicine , Beijing , P.R. China.
2
b Key Laboratory of the Health-Cultivation of the Ministry of Education , Beijing University of Chinese Medicine , Beijing , P.R. China.
3
c Beijing Key Laboratory of the Health-Cultivation , Beijing , P.R. China.
4
d Beijing International Technology Cooperation Base for Prevention and Treatment of Diabetes Mellitus with Chinese Medicine , Beijing , P.R. China.
5
e Department of Science and Technology , Beijing University of Chinese Medicine , Beijing , P.R. China.
6
f Dongfang Hospital Affiliated to Beijing University of Chinese Medicine , Beijing , P.R. China.
7
g School of Chinese Pharmacy , Beijing University of Chinese Medicine , Beijing , P.R. China.
8
h Scientific Research Experiment Center, Beijing University of Chinese Medicine , Beijing , P.R. China.
9
i Beijing Shijitan Hospital, Capital Medical University , Beijing , P.R. China.

Abstract

CONTEXT:

Folium Mori, the leaf of Morus alba L. (Moraceae), has been used in traditional Chinese medicine (TCM) for treating diabetes. However, it is unclear which components in the mulberry leaf are effective for the treatment of type 2 diabetes mellitus (T2DM).

OBJECTIVE:

To investigate the flavonoids and polyphenols in mulberry leaves and their antihyperglycemic and antihyperlipidemic effects in T2DM rats.

MATERIALS AND METHODS:

Male Sprague-Dawley rats were divided into five groups: normal control (NC), diabetic control (DBC), diabetic group with 0.3 mg/kg b.w./day rosiglitazone (RSG), diabetic group with 7 g/kg b.w./day TCM formula and diabetic group with 2 g/kg b.w./day Folium Mori extract (FME). After 4 weeks, the rats were sacrificed; biochemical parameters, gene and protein expression were measured.

RESULTS:

The FBG level was significantly lower in the FME group than in the DBC group (p < 0.05). In oral glucose tolerance test, the AUC was significantly lower in the FME group (p < 0.05). The HOMA-IR level was significantly decreased in the FME group (p < 0.05). FME decreased the total cholesterol (TC), triglyceride (TG) and low density lipoprotein (LDL) levels (p < 0.05). FME increased the mRNA and protein expression of IRS-1, PI3K p85α and Glut-4 increased significantly (p < 0.05). Histological analysis revealed amelioration of lipid accumulation following FME treatment. Additionally, immunohistochemical analysis displayed stronger staining of Glut-4 in the FME group compared to the DBC group.

DISCUSSION AND CONCLUSION:

FME could decrease the body weight, blood glucose, TG, TC and LDL levels, and improve insulin resistance. FME possessed significant antihyperglycemic and antihyperlipidemic activities via the IRS-1/PI3K/Glut-4 signalling pathway.

KEYWORDS:

Antihyperglycemic effect; antihyperlipidemic effect; flavonoids; polyphenols; skeletal muscle

PMID:
27158744
DOI:
10.1080/13880209.2016.1178779
[Indexed for MEDLINE]

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