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Data Brief. 2016 Apr 13;7:1375-82. doi: 10.1016/j.dib.2016.04.018. eCollection 2016 Jun.

A panel data set on harvest and perfusion decellularization of porcine rectus abdominis.

Author information

1
Department of Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, PR China; Department of Regenerative Medicine, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR China.
2
Department of Regenerative Medicine, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR China.
3
Department of Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, PR China.
4
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA.
5
Department of Surgery, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, PR China; Department of Regenerative Medicine, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR China; Department of General Surgery, Shanghai Zhabei District Central Hospital, Shanghai 200072, PR China.
6
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15129, USA.

Abstract

In this dataset, we particularly depicted the harvest and perfusion decellularization of porcine rectus abdominis (RA), accompanied with displaying of the retained vascular trees within the perfusion-decellularized skeletal muscle matrix (pM-ECM) using vascular corrosion casting. In addition, several important tips for successful pM-ECM preparation were emphasized, which including using anatomically isolated skeletal muscle as tissue source with all main feeding and draining vessels perfused, preserving the internal microcirculation availability, aseptic technique and pyrogen free in all steps, sequential perfusion via artery or vein, and longtime washing after decellularization. The data are supplemental to our original research article describing detailed associations of pM-ECM as a clinically relevant scale, three-dimensional scaffold with a vascular network template for tissue-specific regeneration, "Perfusion-decellularized skeletal muscle as a three-dimensional scaffold with a vascular network template" Zhang et al. (2016) [1].

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