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Mater Today Proc. 2016;3(2):206-210.

Bioengineering novel floating nanoparticles for protein and drug delivery.

Author information

1
University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA.
2
University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA ; PuKyong National University, YongSoro 45, Busan 608-737, Korea.
3
University of Maryland, School of Medicine, 701 E. Pratt Street, Baltimore, MD 21202, USA ; University of Baltimore, 1420 N. Charles St., Baltimore, MD 21201, USA.

Abstract

Gas vesicle nanoparticles (GVNPs) are hollow protein nanoparticles produced by Halobacterium sp. NRC-1 which are being engineered for protein delivery. To advance the bioengineering potential of GVNPs, a strain of NRC-1 deleted for the gvpC gene (ΔgvpC) was constructed and a synthetic gene coding for Gaussia princeps luciferase was fused to an abbreviated gvpC gene on an expression plasmid. When introduced into theΔgvpC strain, an active GvpC-luciferase fusion protein bound to GVNPs resulted. These results represent both a technical improvement in the GVNP display system and its expansion for the display of active enzymes.

KEYWORDS:

Gas vesicle naoparticles (GVNPs); Gaussia princeps; enzyme display; luciferase; vaccine

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