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Am J Transl Res. 2016 Feb 15;8(2):698-704. eCollection 2016.

Transformation of tobacco plants by Yali PPO-GFP fusion gene and observation of subcellular localization.

Author information

1
College of Biology Science and Engineering, Hebei University of Economics and Business Shijiazhuang 050061, Hebei, China.
2
Hebei Women's Vocational College Shijiazhuang 050091, Hebei, China.

Abstract

To explore the subcellular localization of Polyphenol oxidase (PPO) from Pyrus bretschneideri, the 1779 bp cDNA of PPO gene excluding the termination codon TAA was cloned and fused with GFP to construct a binary vector pBI121-PPO-GFP. Then, the binary vector was transformed into Nicotiana tabacum by the tumefanciens-mediated method. Using confocal laser scanning microscopy, green fluorescent signals were localized in chloroplasts of the transformed Nicotiana tabacum cell, suggesting that the Polyphenol oxidase from Pyrus bretschneideri was a chloroplast protein.

KEYWORDS:

GFP; PPO; Yali; gene fusion; subcellular localization; transformation

PMID:
27158362
PMCID:
PMC4846919

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