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Am J Transl Res. 2016 Feb 15;8(2):623-33. eCollection 2016.

Elevated serum IL-35 and increased expression of IL-35-p35 or -EBI3 in CD4(+)CD25(+) T cells in patients with active tuberculosis.

Author information

1
Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics No. 1 Xincheng Road, Dongguan 523808, China.
2
Dongguan 6th People's Hospital Dongguan 523008, China.
3
Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsNo. 1 Xincheng Road, Dongguan 523808, China; Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical CollegeNo. 1 Xincheng Road, Dongguan 523808, China.
4
Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical College No. 1 Xincheng Road, Dongguan 523808, China.
5
Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsNo. 1 Xincheng Road, Dongguan 523808, China; Department of Clinical Laboratory, Affiliated Hospital of Guangdong Medical collegeZhanjiang 524001, China.
6
Department of Laboratory Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine Guangzhou 510120, China.
7
Guangdong Provincial Key Laboratory of Medical Molecular DiagnosticsNo. 1 Xincheng Road, Dongguan 523808, China; Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of MedicineChicago 60612, Illinois, United States of America.
8
Department of Medicine, University of Maryland School of Medicine Baltimore, Maryland 21201, United States of America.
9
Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine Chicago 60612, Illinois, United States of America.

Abstract

Despite the recent appreciation of interleukin 35 (IL-35) function in inflammatory diseases, little is known for IL-35 response in patients with active tuberculosis (ATB). In the current study, we demonstrated that ATB patients exhibited increases in serum IL-35 and in mRNA expression of both subunits of IL-35 (p35 and EBI3) in white blood cells and peripheral blood mononuclear cells. Consistently, anti-TB drug treatment led to reduction in serum IL-35 level and p35 or EBI3 expression. TB infection was associated with expression of p35 or EBI3 protein in CD4(+) but not CD8(+) T cells. Most p35(+)CD4(+) T cells and EBI3(+)CD4(+) T cells expressed Treg-associated marker CD25. Our findings may be important in understanding immune pathogenesis of TB. IL-35 in the blood may potentially serve as a biomarker for immune status and prognosis in TB.

KEYWORDS:

CD4+CD25+ T cells; EBI3; Human active pulmonary tuberculosis; interleukin-35; p35

PMID:
27158354
PMCID:
PMC4846911

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