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Biochim Biophys Acta. 2016 Jul;1859(7):871-82. doi: 10.1016/j.bbagrm.2016.05.003. Epub 2016 May 5.

Long non-coding RNA ADNCR suppresses adipogenic differentiation by targeting miR-204.

Author information

1
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
2
United States Department of Agriculture-Agricultural Research Service, Animal Genomics & Improvement Laboratory, Beltsville, MD, United States.
3
Bureau of Animal Husbandry of Biyang County, Biyang, Henan 463700, China.
4
Animal Health Supervision in Henan Province, Zhengzhou, Henan 450008, China. Electronic address: postbai@sohu.com.
5
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: chenhong1212@263.net.

Abstract

Adipogenesis is a complex and precisely orchestrated process mediated by a network of adipogenic regulatory factors. Several studies have highlighted the relevance of lncRNAs in adipocyte differentiation, but the precise molecular mechanism has largely remained elusive. In the present study, we performed Ribo-Zero RNA-Seq to investigate both the poly(A)+and poly(A)-lncRNAs of in vitro cultured bovine preadipocytes and differentiated adipocytes. A stringent set of 2882 lncRNAs was finally identified. A comparison of the lncRNAs expression profiles revealed that 16 lncRNAs are differentially expressed during adipocyte differentiation. We focused on the most downregulated lncRNA, which we named adipocyte differentiation-associated long noncoding RNA (ADNCR). Mechanistically, ADNCR inhibited adipocyte differentiation by functioning as a competing endogenous RNA (ceRNA) for miR-204, thereby augmenting the expression of the miR-204 target gene, SIRT1, which is known to inhibit adipocyte differentiation and adipogenic gene expression by docking with NCoR and SMART to repress PPARγ activity. Our data not only provide a valuable genomic resource for the identification of lncRNAs with functional roles in adipocyte differentiation but also reveal new insights into understanding the mechanisms of adipogenic differentiation.

KEYWORDS:

ADNCR; Adipogenesis; Long noncoding RNAs; Ribo-Zero RNA-Seq; ceRNA

PMID:
27156885
DOI:
10.1016/j.bbagrm.2016.05.003
[Indexed for MEDLINE]

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