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Diagn Microbiol Infect Dis. 2016 Jul;85(3):318-322. doi: 10.1016/j.diagmicrobio.2016.04.011. Epub 2016 Apr 19.

Analytical performance evaluation of Anyplex II HPV28 and Euroarray HPV for genotyping of cervical samples.

Author information

1
Surveillance & Epidemiology of Infectious Diseases, Laboratoire National de Santé, Dudelange, Luxembourg. Electronic address: ardashel.latsuzbaia@lns.etat.lu.
2
Surveillance & Epidemiology of Infectious Diseases, Laboratoire National de Santé, Dudelange, Luxembourg.
3
Department of medicine, Laboratoire National de Santé, Dudelange, Luxembourg.
4
Unit of Cancer Epidemiology/Belgian Cancer Centre, Scientific Institute of Public Health, Brussels, Belgium.
5
Department of Obstetrics and Gynecology, Ghent University Hospital, Ghent, Belgium.

Abstract

Analytically accurate human papillomavirus (HPV) genotyping methods are required to assess the impact of HPV vaccination. The aim of this study was to evaluate the analytical performance of Anyplex II HPV28 (Seegene, Korea) and Euroarray HPV (Euroimmun, Germany) genotyping kits, for conducting a future HPV vaccine efficacy monitoring study in Luxembourg. A total number of 150 cervical swabs were collected from women with mean age 31.4 years. Agreements for detecting any HPV between Aptima/Anyplex (88.0%) and Aptima/Euroarray (90.7%) were similar. Agreement of Anyplex/EuroArray with Aptima was higher for Genotypes 16, 18 or 45 than for the other 11 HPVs. The average number of HPV genotypes detected per sample was similar with 2.6 and 2.5, for Anyplex and EuroArray, respectively. In conclusion, Anyplex and Euroarray showed high agreement in general and in particular for detecting genotypes contained in HPV vaccines.

KEYWORDS:

Anyplex II HPV28; Euroarray HPV; HPV genotyping; Human papilloma virus; Method comparison; Vaccine monitoring

[Indexed for MEDLINE]

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