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Plant Cell Environ. 2017 Jan;40(1):11-24. doi: 10.1111/pce.12764. Epub 2016 Aug 4.

Differential expression of microRNAs and potential targets under drought stress in barley.

Author information

1
Australian Centre for Plant Functional Genomics, PMB1, Glen Osmond, SA, 5064, Australia.
2
School of Agriculture, Food and Wine, The University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.
3
Phenomics and Bioinformatics Research Centre, School of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes, SA, 5095, Australia.
4
The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.
5
ARC Centre of Excellence in Plant Cell Walls, PMB1, Glen Osmond, SA, 5064, Australia.

Abstract

Drought is a crucial environmental constraint limiting crop production in many parts of the world. microRNA (miRNA) based gene regulation has been shown to act in several pathways, including crop response to drought stress. Sequence based profiling and computational analysis have revealed hundreds of miRNAs and their potential targets in different plant species under various stress conditions, but few have been biologically verified. In this study, 11 candidate miRNAs were tested for their expression profiles in barley. Differences in accumulation of only four miRNAs (Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c) were observed between drought-treated and well-watered barley in four genotypes. miRNA targets were predicted using degradome analysis of two, different genotypes, and genotype-specific target cleavage was observed. Inverse correlation of mature miRNA accumulation with miRNA target transcripts was also genotype dependent under drought treatment. Drought-responsive miRNAs accumulated predominantly in mesophyll tissues. Our results demonstrate genotype-specific miRNA regulation under drought stress and evidence for their role in mediating expression of target genes for abiotic stress response in barley.

KEYWORDS:

Hordeum vulgare; canonical cleavage; degradome; genotype; in situ-RT-PCR

PMID:
27155357
DOI:
10.1111/pce.12764
[Indexed for MEDLINE]

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