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J Virol Methods. 2016 Aug;234:160-3. doi: 10.1016/j.jviromet.2016.05.002. Epub 2016 May 4.

Genome characterization of feline morbillivirus from Italy.

Author information

1
Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
2
Faculty of Veterinary Medicine, University of Teramo, Italy.
3
Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy. Electronic address: a.lorusso@izs.it.

Abstract

Feline morbillivirus (FeMV) has been recently identified by RT-PCR in the urine sample of a nephropathic cat in Italy. In this report, we describe the whole genome sequence of strain Piuma/2015 obtained by combination of sequence independent single primer amplification method (SISPA) and next generation sequencing (NGS) starting from RNA purified from the infected urine sample. The existence in Germany and Turkey of FeMVs from cats divergent from Piuma/2015, suggests the presence of FeMV heterogeneity in Europe as it has been described previously in Japan and China.

KEYWORDS:

Cat; Feline morbillivirus; Italy; NGS; SISPA; Urine

PMID:
27155238
DOI:
10.1016/j.jviromet.2016.05.002
[Indexed for MEDLINE]

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