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Biosens Bioelectron. 2016 Nov 15;85:32-45. doi: 10.1016/j.bios.2016.04.091. Epub 2016 Apr 27.

Aptamers, antibody scFv, and antibody Fab' fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements.

Author information

1
Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada.
2
Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada. Electronic address: mikethom@chem.utoronto.ca.

Abstract

The choice of biosensing elements is crucial for the development of the optimal biosensor. Three of the most versatile biosensing elements are antibody single-chain Fv fragments (scFv), antibody fragment-antigen binding (Fab') units, and aptamers. This article provides an overview of these three biorecognition elements with respects to their synthesis/engineering, various immobilization techniques, and examples of their use in biosensors. Furthermore, the final section of the review compares and contrasts their characteristics (time/cost of development, ease and variability of immobilization, affinity, stability) illustrating their advantages and disadvantages. Overall, scFv fragments are found to display the highest customizability (i.e. addition of functional groups, immobilizing peptides, etc.) due to recombinant synthesis techniques. If time and cost are an issue in the development of the biosensor, Fab' fragments should be chosen as they are relatively cheap and can be developed quickly from whole antibodies (several days). However, if there are sufficient funds and time is not a factor, aptamers should be utilized as they display the greatest affinity towards their target analytes and are extremely stable (excellent biosensor regenerability).

KEYWORDS:

Aptamers; Biorecognition elements; Biosensors; Fab' fragments; ScFv fragments

PMID:
27155114
DOI:
10.1016/j.bios.2016.04.091
[Indexed for MEDLINE]

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