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Gene. 2016 Aug 10;587(2):120-31. doi: 10.1016/j.gene.2016.04.060. Epub 2016 May 3.

Transcriptomic analysis of Arabidopsis overexpressing flowering locus T driven by a meristem-specific promoter that induces early flowering.

Author information

1
Departamento de Biotecnología y Bioingenieria, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508 San Pedro Zacatenco, 07360 Mexico City, Mexico.
2
Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.
3
Departamento de Biotecnología y Bioingenieria, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508 San Pedro Zacatenco, 07360 Mexico City, Mexico. Electronic address: bxoconos@cinvestav.mx.

Abstract

Here we analyzed in leaves the effect of FT overexpression driven by meristem-specific KNAT1 gene homolog of Arabidopsis thaliana (Lincoln et al., 1994; Long et al., 1996) on the transcriptomic response during plant development. Our results demonstrated that meristematic FT overexpression generates a phenotype with an early flowering independent of photoperiod when compared with wild type (WT) plants. Arabidopsis FT-overexpressor lines (AtFTOE) did not show significant differences compared with WT lines neither in leaf number nor in rosette diameter up to day 21, when AtFTOE flowered. After this period AtFTOE plants started flower production and no new rosette leaves were produced. Additionally, WT plants continued on vegetative stage up to day 40, producing 12-14 rosette leaves before flowering. Transcriptomic analysis of rosette leaves studied by sequencing Illumina RNA-seq allowed us to determine the differential expression in mature leaf rosette of 3652 genes, being 626 of them up-regulated and 3026 down-regulated. Overexpressed genes related with flowering showed up-regulated transcription factors such as MADS-box that are known as flowering markers in meristem and which overexpression has been related with meristem identity preservation and the transition from vegetative to floral stage. Genes related with sugar transport have shown a higher demand of monosaccharides derived from the hydrolysis of sucrose to glucose and probably fructose, which can also be influenced by reproductive stage of AtFTOE plants.

KEYWORDS:

Arabidopsis; Flowering locus T; Plant development; RNA-seq analysis; Transgenic plants

PMID:
27154816
PMCID:
PMC4911706
DOI:
10.1016/j.gene.2016.04.060
[Indexed for MEDLINE]
Free PMC Article

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