Format

Send to

Choose Destination
Neuron. 2016 May 4;90(3):564-80. doi: 10.1016/j.neuron.2016.04.020.

Two Clathrin Adaptor Protein Complexes Instruct Axon-Dendrite Polarity.

Author information

1
Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, CA 94305, USA.
2
Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
3
Howard Hughes Medical Institute, Department of Biology, Stanford University, Stanford, CA 94305, USA. Electronic address: kangshen@stanford.edu.

Abstract

The cardinal feature of neuronal polarization is the establishment and maintenance of axons and dendrites. How axonal and dendritic proteins are sorted and targeted to different compartments is poorly understood. Here, we identified distinct dileucine motifs that are necessary and sufficient to target transmembrane proteins to either the axon or the dendrite through direct interactions with the clathrin-associated adaptor protein complexes (APs) in C. elegans. Axonal targeting requires AP-3, while dendritic targeting is mediated by AP-1. The axonal dileucine motif binds to AP-3 with higher efficiency than to AP-1. Both AP-3 and AP-1 are localized to the Golgi but occupy adjacent domains. We propose that AP-3 and AP-1 directly select transmembrane proteins and target them to axon and dendrite, respectively, by sorting them into distinct vesicle pools.

PMID:
27151641
PMCID:
PMC5266526
DOI:
10.1016/j.neuron.2016.04.020
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center