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Prenat Diagn. 2016 Jul;36(7):672-9. doi: 10.1002/pd.4837. Epub 2016 Jun 7.

Noninvasive prenatal testing (NIPT) in twin pregnancies with treatment of assisted reproductive techniques (ART) in a single center.

Tan Y1,2,3, Gao Y4,5, Lin G1,2,3, Fu M6, Li X3, Yin X4,5, Du J1,2,3, Li J7, Li W1,2,3, Peng H7, Yuan Y6, Chen F4,5,8, Jiang F6, Zhang H6, Lu G2,3, Gong F1,2,3, Wang W4,6.

Author information

Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, China.
Key Laboratory of Reproductive and Stem Cell Engineering, Ministry of Health, Changsha, China.
Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, China.
BGI-Shenzhen, Shenzhen, China.
China National Genebank-Shenzhen, BGI-Shenzhen, Shenzhen, China.
Clinical laboratory of BGI Health, BGI-Shenzhen, Shenzhen, China.
Clinical Laboratory of BGI Health, BGI-Wuhan, Wuhan, China.
Section of Molecular Disease Biology, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.



The objective of the study is to report the performance of noninvasive prenatal testing (NIPT) in twin pregnancies after the treatment of assisted reproductive technology (ART).


In two years period, 565 pregnant women with ART twin pregnancies were prospectively tested by NIPT for screening for trisomy 21 (T21), 18 (T18), and 13 (T13) by sequencing cell-free DNA in maternal plasma. Positive NIPT results were confirmed by karyotyping, while negative results were interviewed after delivery. Pregnant decision based on NIPT and confirmation results was discussed during post-test counseling.


In total of 565 cases, NIPT had a failure rate of 0.9% (5/565). Four cases of T21 were identified by NIPT and confirmed by karyotyping, resulting in 100% (95%CI 39.8%-100%) positive predictive value. Among 556 cases with NIPT negative results, 506 cases (91.0%) were confirmed by follow-up of postnatal phenotypes, while 33 cases (5.9%) had adverse pregnant outcomes with unconfirmed reasons because of the lack of cytogenetic samples. The remaining 17 cases (3.1%) refused follow-up. No false negative result was reported.


With apparently high positive predictive value and low false positive rate, NIPT has the potential to be used as a good alternative approach of conventional prenatal screening at the first trimester in ART twin pregnancy. © 2016 John Wiley & Sons, Ltd.

[Indexed for MEDLINE]

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