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RNA Biol. 2016 Jun 2;13(6):593-604. doi: 10.1080/15476286.2016.1184384. Epub 2016 May 5.

Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing.

Author information

1
a Institute of Plant Biology, National Taiwan University , Taipei , Taiwan.
2
b Institute of Plant and Microbial Biology, Academia Sinica, Nankang , Taipei , Taiwan.
3
c Biotechnology Center, National Chung-Hsing University , Taichung , Taiwan.

Abstract

The mitochondrion is an important power generator in most eukaryotic cells. To preserve its function, many essential nuclear-encoded factors play specific roles in mitochondrial RNA metabolic processes, including RNA editing. RNA editing consists of post-transcriptional deamination, which alters specific nucleotides in transcripts to mediate gene expression. In plant cells, many pentatricopeptide repeat proteins (PPRs) participate in diverse organellar RNA metabolic processes, but only PLS-type PPRs are involved in RNA editing. Here, we report a P-type PPR protein from Arabidopsis thaliana, P-type PPR-Modulating Editing (PPME), which has a distinct role in mitochondrial nad1 RNA editing via RNA binding activity. In the homozygous ppme mutant, cytosine (C)-to-uracil (U) conversions at both the nad1-898 and 937 sites were abolished, disrupting Arg(300)-to-Trp(300) and Pro(313)-to-Ser(313) amino acid changes in the mitochondrial NAD1 protein. NAD1 is a critical component of mitochondrial respiration complex I; its activity is severely reduced in the homozygous ppme mutant, resulting in significantly altered growth and development. Both abolished RNA editing and defective complex I activity were completely rescued by CaMV 35S promoter- and PPME native promoter-driven PPME genomic fragments tagged with GFP in a homozygous ppme background. Our experimental results demonstrate a distinct role of a P-type PPR protein, PPME, in RNA editing in plant organelles.

KEYWORDS:

Arabidopsis; PPR; complex I activity; editing; mitochondria; nad1

PMID:
27149614
PMCID:
PMC4962808
DOI:
10.1080/15476286.2016.1184384
[Indexed for MEDLINE]
Free PMC Article

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