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Cytopathology. 2016 Oct;27(5):359-68. doi: 10.1111/cyt.12323. Epub 2016 May 5.

Guidelines for May-Grünwald-Giemsa staining in haematology and non-gynaecological cytopathology: recommendations of the French Society of Clinical Cytology (SFCC) and of the French Association for Quality Assurance in Anatomic and Cytologic Pathology (AFAQAP).

Author information

1
Centre de Pathologie Est, Hospices Civils de Lyon, Université Claude Bernard Lyon 1, Bron, France. eric.piaton@chu-lyon.fr.
2
Société Française de Cytologie Clinique (SFCC), Paris, France. eric.piaton@chu-lyon.fr.
3
Société Française de Cytologie Clinique (SFCC), Paris, France.
4
Service d'Anatomie et Cytologie Pathologiques, Hôpital Universitaire Necker Enfants Malades, Université Paris Descartes, Paris, France.
5
Laboratoire d'Anatomo-Pathologie, Centre Hospitalier René Dubos, Cergy Pontoise, France.
6
Cabinet de Pathologie, Strasbourg, France.
7
Département d'Anatomie et Cytologie Pathologiques, IUC Toulouse Oncopôle, Toulouse, France.
8
Unité de Cytopathologie, Gustave Roussy, Villejuif, France.
9
Service d'Anatomie et Cytologie Pathologiques, Hôpital Haut-Lévêque (CHU de Bordeaux), Pessac, France.
10
Centre de Pathologie Est, Hospices Civils de Lyon, Université Claude Bernard Lyon 1, Bron, France.
11
UF de Cytologie Pathologique, Institut de Biologie et de Pathologie, CHU de Grenoble, Grenoble, France.
12
Cabinet de Pathologie, SCP des Drs Bloget et Declerck, Avon, France.
13
Service d'Anatomie et Cytologie Pathologiques, Hôpital Tenon, Paris, France.
14
Association Française d'Assurance Qualité en Anatomie et Cytologie Pathologiques (AFAQAP), Strasbourg, France.
15
Département de Pathologie, Hôpital de Hautepierre, CHU de Strasbourg, Strasbourg, France.
16
Service d'Anatomie et Cytologie Pathologiques, Hôpital Pasteur, CHU de Nice, Nice, France.
17
Service d'Anatomie et Cytologie Pathologiques, Université Paris Descartes, Hôpitaux Universitaires Paris Centre, site Cochin, AP-HP, Paris, France.

Abstract

OBJECTIVE:

Since the guidelines of the International Committee for Standardisation in Haematology (ICSH) in 1984 and those of the European Committee for External Quality Assessment Programmes in Laboratory Medicine (EQALM) in 2004, no leading organisation has published technical recommendations for the preparation of air-dried cytological specimens using May-Grünwald-Giemsa (MGG) staining.

DATA SOURCES:

Literature data were retrieved using reference books, baseline-published studies, articles extracted from PubMed/Medline and Google Scholar, and online-available industry datasheets.

RATIONALE:

The present review addresses all pre-analytical issues concerning the use of Romanowsky's stains (including MGG) in haematology and non-gynaecological cytopathology. It aims at serving as actualised, best practice recommendations for the proper handling of air-dried cytological specimens. It, therefore, appears complementary to the staining criteria of the non-gynaecological diagnostic cytology handbook edited by the United Kingdom National External Quality Assessment Service (UK-NEQAS) in February 2015.

KEYWORDS:

Giemsa; May-Grünwald; Romanowsky's stains; Wright; non-gynaecological cytopathology; quality assurance

PMID:
27146425
DOI:
10.1111/cyt.12323
[Indexed for MEDLINE]

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