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Neuron. 2016 May 4;90(3):492-8. doi: 10.1016/j.neuron.2016.03.013. Epub 2016 Apr 14.

Fast, Temperature-Sensitive and Clathrin-Independent Endocytosis at Central Synapses.

Author information

1
Carl-Ludwig-Institute for Physiology, Medical Faculty, University of Leipzig, Liebigstrasse 27, 04103 Leipzig, Germany. Electronic address: igor.delvendahl@medizin.uni-leipzig.de.
2
The Vollum Institute, Oregon Health & Science University, Portland, OR 97239, USA.
3
Carl-Ludwig-Institute for Physiology, Medical Faculty, University of Leipzig, Liebigstrasse 27, 04103 Leipzig, Germany. Electronic address: hallermann@medizin.uni-leipzig.de.

Abstract

The fusion of neurotransmitter-filled vesicles during synaptic transmission is balanced by endocytotic membrane retrieval. Despite extensive research, the speed and mechanisms of synaptic vesicle endocytosis have remained controversial. Here, we establish low-noise time-resolved membrane capacitance measurements that allow monitoring changes in surface membrane area elicited by single action potentials and stronger stimuli with high-temporal resolution at physiological temperature in individual bona-fide mature central synapses. We show that single action potentials trigger very rapid endocytosis, retrieving presynaptic membrane with a time constant of 470 ms. This fast endocytosis is independent of clathrin but mediated by dynamin and actin. In contrast, stronger stimuli evoke a slower mode of endocytosis that is clathrin, dynamin, and actin dependent. Furthermore, the speed of endocytosis is highly temperature dependent with a Q10 of ∼3.5. These results demonstrate that distinct molecular modes of endocytosis with markedly different kinetics operate at central synapses.

PMID:
27146271
PMCID:
PMC5125781
DOI:
10.1016/j.neuron.2016.03.013
[Indexed for MEDLINE]
Free PMC Article

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