Format

Send to

Choose Destination
Immunopharmacol Immunotoxicol. 2016 Jun;38(3):228-37. doi: 10.3109/08923973.2016.1173058.

Anti-oxidant and anti-inflammatory effects of apigenin in a rat model of sepsis: an immunological, biochemical, and histopathological study.

Author information

1
a Department of Microbiology, Faculty of Medicine , Kafkas University , Kars , Turkey ;
2
b Department of Biochemistry, Faculty of Veterinary Medicine , Ataturk University , Erzurum , Turkey ;
3
c Department of Pathology, Faculty of Medicine , Ataturk University , Erzurum , Turkey ;
4
d Department of Pathology, Faculty of Medicine , Kafkas University , Kars , Turkey ;
5
e Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine , Kafkas University , Kars , Turkey ;
6
f Department of Histology and Embryology, Faculty of Medicine , Kafkas University , Kars , Turkey.

Abstract

OBJECTIVE:

We hypothesize that apigenin may inhibit some cellular process of sepsis-induced spleen injury and simultaneously improve inflammation and oxidative stress. Therefore, the aim of this study was to investigate the potential protective effects of apigenin in a polymicrobial sepsis rat model of by cecal ligation and puncture.

MATERIALS AND METHODS:

64 female Wistar albino rats were divided into 8 groups. The pro-inflammatory (tumor necrosis factor-alpha, interleukin-6, and interleukin-1-beta) and anti-inflammatory (tumor growth factor-beta and interleukin-10) cytokine levels were measured by enzyme-linked immunosorbent assay. CD3, CD68, and nuclear factor kappa B (NF-κB) positivity rates were detected by immunohistochemical methods. Oxidative stress parameters were measured by tissue biochemistry.

RESULTS:

Sepsis caused a significant increase in TNF-alpha, IL-1-beta, IL-6, and TGF-beta levels whereas it reduced IL-10 level. Additionally, it led to an increase in CD3, CD68, and NF-κB positivity rates as well as oxidative stress parameters levels. However, apigenin inhibited the inflammation process, increased the IL-10 level and normalized the oxidative stress parameters.

DISCUSSION AND CONCLUSION:

Pretreatment with apigenin results in a significant reduction in the amount of inflammatory cells. The beneficial effect of apigenin on spleen injury also involved inhibition of NF-κB pathway, suppression of proinflammatory cytokines, and induction of anti-inflammatory cytokine production. Additionally, it led to a decrease in oxidative stress in spleen tissue. Taking everything into account, apigenin may be an alternative therapeutic option for prevention of sepsis-induced organ.

KEYWORDS:

Apigenin; CD3 and CD68; CLP sepsis; NF-κB; cytokines; oxidative stress

PMID:
27144896
DOI:
10.3109/08923973.2016.1173058
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center