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Int J Mol Sci. 2016 Apr 30;17(5). pii: E656. doi: 10.3390/ijms17050656.

Ewing's Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue.

Author information

1
Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. antonina.parafioriti@gpini.it.
2
Dipartimento di Medicina, Sezione di Medicina Interna B, Università di Verona, Verona 37134, Italy. caterina.bason@univr.it.
3
Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. elisabetta.armiraglio@asst-pini-cto.it.
4
Dipartimento di Sanità Pubblica e Medicina di Comunità, Sezione di Epidemiologia e Statistica Medica, Università di Verona, Verona 37134, Italy. lucia.calciano@univr.it.
5
Unità Operativa Complessa (U.O.C.) Chirurgia Ortopedica Oncologica, Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. PrimoAndrea.Daolio@asst-pini-cto.it.
6
Unità Operativa Complessa (U.O.C.) Immunoematologia-Medicina Trasfusionale e Laboratorio di Ematologia, Laboratorio di Ricerca "Cellule Staminali" Azienda Unità Sanitaria Locale (AUSL)-Ospedale Santo Spirito, Pescara 65125, Italy. martina.berardocco@gmail.com.
7
Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. andrea.dibernardo@asst-pini-cto.it.
8
Facoltà di Medicina Veterinaria, Università di Teramo, Teramo 64100, Italy. acolosimo@unite.it.
9
Dipartimento di Oncologia Pediatrica, Fondazione-Istituto di Ricovero e Cura a Carattere Scientifico-(IRCCS) Istituto Nazionale dei Tumori, Milano 20133, Italy. Roberto.Luksch@istitutotumori.mi.it.
10
Unità Operativa Complessa (U.O.C.) Azienda Socio Sanitaria Territoriale Centro Specialistico Ortopedico Traumatologico Gaetano Pini-CTO, Milano 20122, Italy. annacberardi@yahoo.it.
11
Unità Operativa Complessa (U.O.C.) Immunoematologia-Medicina Trasfusionale e Laboratorio di Ematologia, Laboratorio di Ricerca "Cellule Staminali" Azienda Unità Sanitaria Locale (AUSL)-Ospedale Santo Spirito, Pescara 65125, Italy. annacberardi@yahoo.it.

Abstract

The molecular mechanism responsible for Ewing's Sarcoma (ES) remains largely unknown. MicroRNAs (miRNAs), a class of small non-coding RNAs able to regulate gene expression, are deregulated in tumors and may serve as a tool for diagnosis and prediction. However, the status of miRNAs in ES has not yet been thoroughly investigated. This study compared global miRNAs expression in paraffin-embedded tumor tissue samples from 20 ES patients, affected by primary untreated tumors, with miRNAs expressed in normal human mesenchymal stromal cells (MSCs) by microarray analysis. A miRTarBase database was used to identify the predicted target genes for differentially expressed miRNAs. The miRNAs microarray analysis revealed distinct patterns of miRNAs expression between ES samples and normal MSCs. 58 of the 954 analyzed miRNAs were significantly differentially expressed in ES samples compared to MSCs. Moreover, the qRT-PCR analysis carried out on three selected miRNAs showed that miR-181b, miR-1915 and miR-1275 were significantly aberrantly regulated, confirming the microarray results. Bio-database analysis identified BCL-2 as a bona fide target gene of the miR-21, miR-181a, miR-181b, miR-29a, miR-29b, miR-497, miR-195, miR-let-7a, miR-34a and miR-1915. Using paraffin-embedded tissues from ES patients, this study has identified several potential target miRNAs and one gene that might be considered a novel critical biomarker for ES pathogenesis.

KEYWORDS:

Ewing’s Sarcoma; human mesenchymal stem cells; miRTarBase database; microRNAs

PMID:
27144561
PMCID:
PMC4881482
DOI:
10.3390/ijms17050656
[Indexed for MEDLINE]
Free PMC Article

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