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Cell Mol Life Sci. 2016 Nov;73(21):4101-20. doi: 10.1007/s00018-016-2239-5. Epub 2016 May 3.

GFAP isoforms control intermediate filament network dynamics, cell morphology, and focal adhesions.

Author information

1
Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands.
2
Soft Tissue Biomechanics & Engineering, Department of biomedical engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
3
Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands.
4
Physics of Life Processes, Leiden Institute of Physics, Leiden, The Netherlands.
5
Cell Biology and Histology, AMC Medical Center, Amsterdam, The Netherlands.
6
Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands. e.m.hol-2@umcutrecht.nl.
7
Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG, Utrecht, The Netherlands. e.m.hol-2@umcutrecht.nl.
8
Swammerdam Institute for Life Sciences, Center for Neuroscience, University of Amsterdam, Amsterdam, The Netherlands. e.m.hol-2@umcutrecht.nl.

Abstract

Glial fibrillary acidic protein (GFAP) is the characteristic intermediate filament (IF) protein in astrocytes. Expression of its main isoforms, GFAPα and GFAPδ, varies in astrocytes and astrocytoma implying a potential regulatory role in astrocyte physiology and pathology. An IF-network is a dynamic structure and has been functionally linked to cell motility, proliferation, and morphology. There is a constant exchange of IF-proteins with the network. To study differences in the dynamic properties of GFAPα and GFAPδ, we performed fluorescence recovery after photobleaching experiments on astrocytoma cells with fluorescently tagged GFAPs. Here, we show for the first time that the exchange of GFP-GFAPδ was significantly slower than the exchange of GFP-GFAPα with the IF-network. Furthermore, a collapsed IF-network, induced by GFAPδ expression, led to a further decrease in fluorescence recovery of both GFP-GFAPα and GFP-GFAPδ. This altered IF-network also changed cell morphology and the focal adhesion size, but did not alter cell migration or proliferation. Our study provides further insight into the modulation of the dynamic properties and functional consequences of the IF-network composition.

KEYWORDS:

Astrocytoma; FRAP; GFAP; Intermediate filaments

PMID:
27141937
PMCID:
PMC5043008
DOI:
10.1007/s00018-016-2239-5
[Indexed for MEDLINE]
Free PMC Article

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