Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2016 May 17;113(20):5706-11. doi: 10.1073/pnas.1523899113. Epub 2016 May 2.

Bacteriocin from epidemic Listeria strains alters the host intestinal microbiota to favor infection.

Author information

1
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France;
2
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France; Cellule Pasteur, Université Paris Diderot, Sorbonne Paris Cité, Paris F-75015, France;
3
Bioinformatics and Biostatistics Hub - Centre de Bioinformatique, Biostatistique et Biologie Intégrative, Unité mixte de Service et Recherche 3756 Institut Pasteur- Centre National de la Recherche Scientifique, Paris F-75015, France;
4
Biomics Pole, Centre d'Innovation et Recherche Technologique, Institut Pasteur, Paris F-75015, France;
5
Institut National de la Recherche Agronomique, Unité Mixte de Recherché 1319 Micalis, Jouy-en-Josas F-78350, France; AgroParisTech, Unité Mixte de Recherché 1319 Micalis, Jouy-en-Josas F-78350, France.
6
Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris F-75015, France; Institut National de la Santé et de la Recherche Médicale, U604, Paris F-75015, France; Institut National de la Recherche Agronomique, Unite Sous Contrat 2020, Paris F-75015, France; javier.pizarro-cerda@pasteur.fr.

Abstract

Listeria monocytogenes is responsible for gastroenteritis in healthy individuals and for a severe invasive disease in immunocompromised patients. Among the three identified L. monocytogenes evolutionary lineages, lineage I strains are overrepresented in epidemic listeriosis outbreaks, but the mechanisms underlying the higher virulence potential of strains of this lineage remain elusive. Here, we demonstrate that Listeriolysin S (LLS), a virulence factor only present in a subset of lineage I strains, is a bacteriocin highly expressed in the intestine of orally infected mice that alters the host intestinal microbiota and promotes intestinal colonization by L. monocytogenes, as well as deeper organ infection. To our knowledge, these results therefore identify LLS as the first bacteriocin described in L. monocytogenes and associate modulation of host microbiota by L. monocytogenes epidemic strains to increased virulence.

KEYWORDS:

Listeria; bacteriocin; epidemic; infection; intestinal microbiota

PMID:
27140611
PMCID:
PMC4878514
DOI:
10.1073/pnas.1523899113
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center