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J Ethnopharmacol. 2016 Jul 21;188:266-74. doi: 10.1016/j.jep.2016.04.057. Epub 2016 Apr 30.

Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages.

Author information

1
Programas Multidisciplinarios de Posgrado en Ciencias Ambientales (PMPCA) de la Universidad Autónoma de San Luis Potosí, S. L. P., México; Department Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, IL 61801, USA.
2
Programas Multidisciplinarios de Posgrado en Ciencias Ambientales (PMPCA) de la Universidad Autónoma de San Luis Potosí, S. L. P., México; Instituto de Investigación de Zonas Desérticas (IIZD) de la Universidad Autónoma de San Luis Potosí, S. L. P., México.
3
United States Department of Agriculture, Agricultural Research Service, 1815 North University Street, Peoria, IL 61604, USA.
4
Department Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, IL 61801, USA. Electronic address: edemejia@illinois.edu.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Calea urticifolia leaves are traditionally used as a remedy to treat gastric ulcers, diabetes, and inflammation by the Xi'uy ancient native community of San Luis Potosi, Mexico.

AIM OF THE STUDY:

The aim was to assess the effects of the aqueous extract of the Mexican plant C. urticifolia as anti-inflammatory and anti-oxidant using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and to provide evidence on the phenolic compounds.

MATERIALS AND METHODS:

RAW 264.7 macrophages were stimulated with 1µg/mL of LPS and treated with 10, 25 50, 75 y 100µg/mL of Calea urticifolia lyophilized aqueous extract (CuAqE). Nitric oxide (NO) release, tumor necrosis factor alpha, prostaglandin E2 production, inducible nitric oxide synthase (iNOS), cyclooxygenase-2, nuclear factor-κB (NF-κB) p65, NF-κB p50 expression and reactive oxygen species (ROS) were measured; other pro-inflammatory proteins were measured with membrane antibody array. Phenolic compounds were analyzed by LC-ESI-MS.

RESULTS:

Inflammation was inhibited by suppressing iNOS/NO pathway through inhibiting nucleus translocation of NF-κB p65 and p50 sub-units. ROS production was significantly (P<0.05) inhibited in a dose-dependent manner in LPS-stimulated macrophages. Moreover, the expression of inflammatory markers was suppressed (34.5-88.3%) by CuAqE. A mix of caffeoylquinic acid derivatives and flavonoid-glycosides were found in CuAqE.

CONCLUSION:

Phenolic compounds in CuAqE such as caffeoylquinic acid derivatives and flavonoid glycosides could be responsible for inhibiting LPS-induced inflammation and oxidative stress by iNOS/NO pathway through suppressing NF-κB signaling pathway and by inhibition of ROS production in RAW 264.7 macrophages. Therefore, these results support the traditional knowledge of C. urticifolia tea such as an anti-inflammatory and antioxidant agent.

KEYWORDS:

Calea urticifolia; Inflammation; NF-κB; Nitric oxide; Phenolic compounds; Reactive oxygen species

PMID:
27139571
DOI:
10.1016/j.jep.2016.04.057
[Indexed for MEDLINE]

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