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Plant J. 2016 Aug;87(3):270-80. doi: 10.1111/tpj.13198. Epub 2016 Jul 6.

Transfer RNA maturation in Chlamydomonas mitochondria, chloroplast and the nucleus by a single RNase P protein.

Author information

1
Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France.
2
Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 12 Rue du Général Zimmer, 67000, Strasbourg, France. giege@unistra.fr.

Abstract

The maturation of tRNA precursors involves the 5' cleavage of leader sequences by an essential endonuclease called RNase P. Beyond the ancestral ribonucleoprotein (RNP) RNase P, a second type of RNase P called PRORP (protein-only RNase P) evolved in eukaryotes. The current view on the distribution of RNase P in cells is that multiple RNPs, multiple PRORPs or a combination of both, perform specialised RNase P activities in the different compartments where gene expression occurs. Here, we identify a single gene encoding PRORP in the green alga Chlamydomonas reinhardtii while no RNP is found. We show that its product, CrPRORP, is triple-localised to mitochondria, the chloroplast and the nucleus. Its downregulation results in impaired tRNA biogenesis in both organelles and the nucleus. CrPRORP, as a single-subunit RNase P for an entire organism, makes up the most compact and versatile RNase P machinery described in either prokaryotes or eukaryotes.

KEYWORDS:

Chlorophyta; RNase P; evolution; protein-only RNase P; tRNA biogenesis

PMID:
27133210
DOI:
10.1111/tpj.13198
[Indexed for MEDLINE]
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