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J Infect Dis. 2016 Aug 1;214(3):403-7. doi: 10.1093/infdis/jiw172. Epub 2016 Apr 30.

Diversity and Tropism of HIV-1 Rebound Virus Populations in Plasma Level After Treatment Discontinuation.

Author information

1
Lineberger Comprehensive Cancer Center.
2
Departments of Medicine and Neuroscience Center of Translational AIDS Research, Lewis Katz School of Medicine at Temple University.
3
Division of Infectious Diseases.
4
Perelman School of Medicine, University of Pennsylvania, Philadelphia.
5
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill.

Abstract

Human immunodeficiency virus-infected people discontinuing therapy experience a rebound in the virus level (hereafter, "rebound virus") from a persistent reservoir. We examined 10 samples from patients in AIDS Clinical Trials Group study A5068 with rebound virus, using single-genome amplification and Primer ID deep sequencing, to assess env genetic diversity of the virus population. Most rebound-virus populations showed significant diversity. All env examined required high levels of CD4 for entry, consistent with selection of replication in CD4(+) T cells. These results indicate that most people discontinuing therapy release a diverse population of virus and that this released virus has entry features of virus selected for replication in CD4(+) T cells, rather than in myeloid cells.

KEYWORDS:

HIV-1; genetic diversity; rebound virus; reservoir; tropism

PMID:
27132284
PMCID:
PMC4936648
DOI:
10.1093/infdis/jiw172
[Indexed for MEDLINE]
Free PMC Article

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